Analysis of plasma histamine: a modification of the enzymatic isotopic assay

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. Allergy Clin. Immunol.; (United States)

DOI:https://doi.org/10.1016/0091-6749(86)90407-0· OSTI ID:5562897

Rauls, D O; Ting, S; Lund, M

The enzymatic isotopic analysis of histamine requires extraction and concentration of (/sup 3/H)-1-methylhistamine from the reaction mixture. The assay was modified to include enzymatic reaction at 0 degrees C to reduce blank values and direct application of protein-free reaction mixture to a thin-layer chromatography plate for isolating of the product. The analysis was quantitative down to 100 pg/ml of histamine, adequate for monitoring plasma histamine content.

• Research Organization: William Beaumont Army Medical Center, El Paso, TX
• OSTI ID: 5562897
• Journal Information: J. Allergy Clin. Immunol.; (United States), Journal Name: J. Allergy Clin. Immunol.; (United States) Vol. 5; ISSN JACIB
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AZOLES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOENZYMATIC ASSAY
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS