Analysis of human HPRT deletion mutations with X-linked probes and pulsed field gel electrophoresis
Journal Article
·
· Environmental and Molecular Mutagenesis; (United States)
- Univ. of Vermont, Burlington (United States)
Because the human hprt gene is used in numerous mutation studies, it is important to fully characterize this gene. Therefore, the authors laboratory has undertaken to map the region around the hprt gene at band q26 of the human X chromosome. Utilizing hprt mutant T-cell clones isolated using the hprt clonal assay, which have deletions of all of part of the hprt gene, the authors have ordered 5 anonymous probes previously known to map in Xq26. Results suggest that this region include between 460 kb and 18 Mb of DNA, which is at least 10 times the size of the hprt gene itself (43 kb). Pulsed field gel analysis of the region is underway to determine the exact distances between each of the anonymous probes and hprt and to determine deletion sizes in the mutant T-cell clones.
- OSTI ID:
- 5560623
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:4; ISSN EMMUE; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
GENE MUTATIONS
GLYCOSYL TRANSFERASES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MOLECULAR BIOLOGY
MUTATIONS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
X CHROMOSOME
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
GENE MUTATIONS
GLYCOSYL TRANSFERASES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MOLECULAR BIOLOGY
MUTATIONS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
X CHROMOSOME