Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy

Journal Article · Sat Feb 01 00:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.3.1138· OSTI ID:5559487

Lynch, C M; Miller, A D ^[1]; Clowes, M M; Osborne, W R.A.; Clowes, A W ^[2]

---

1. Fred Hutchinson Cancer Research Center, Seattle, WA (United States)
2. Univ. of Washington, Seattle (United States)

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli {beta}-galactosidase gene or a human adenosine deaminase gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.

• OSTI ID: 5559487
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:3; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDOTHELIUM
GENE RECOMBINATION
NON-PEPTIDE C-N HYDROLASES
GENE REGULATION
ARTERIES
ENZYME IMMUNOASSAY
MUSCLES
RATS
THERAPY
ANIMAL TISSUES
ANIMALS
BIOASSAY
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
ENZYMES
HYDROLASES
IMMUNOASSAY
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques