Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure
- Univ. of Illinois, Urbana (United States)
The authors have obtained proton-coupled carbon-13 nuclear magnetic resonance (NMR) spectra of a variety of lipid-water and lipid-drug-water systems, at 11.7 T, as a function of temperature, using the 'magic-angle' sample-spinning (MAS) NMR technique. The resulting spectra show a wide range of line shapes, due to interferences between dipole-dipole and dipole-chemical shielding anisotropy interactions. The differential line-broadening effects observed are particularly large for aromatic and olefinic (sp{sup 2}) carbon atom sites. Coupled spectra of the tricyclic antidepressants desipramine and imipramine, in 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophases, show well-resolved doublets having different line shapes for each of the four aromatic methine groups, due to selective averaging of the four C-H dipolar interactions due to rapid motion about the director (or drug C{sub 2}) axis. {sup 2}H NMR spectra of (2,4,6,8-{sup 2}H{sub 4})desipramine (and imipramine) in the same 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophase exhibit quadrupole splittings of {approximately}0-2 and {approximately}20 kHz, indicating an approximate magic-angle orientation of the C2-{sup 2}H({sup 1}H) and C8-{sup 2}H({sup 1}H) vectors with respect to an axis of motional averaging, in accord with the {sup 13}C NMR results. The good qualitative agreement between {sup 13}C and {sup 2}H NMR results suggests that useful orientational ({sup 2}H NMR like) information can be deduced from natural-abundance {sup 13}C NMR spectra of a variety of mobile solids.
- OSTI ID:
- 5559189
- Journal Information:
- Biochemistry; (United States), Vol. 30:46; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIDEPRESSANTS
BIOLOGICAL EFFECTS
CELL MEMBRANES
MOLECULAR STRUCTURE
LIPIDS
NUCLEAR MAGNETIC RESONANCE
CARBON 13
DEUTERIUM COMPOUNDS
PHOSPHOLIPIDS
PROTONS
BARYONS
CARBON ISOTOPES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
ELEMENTARY PARTICLES
ESTERS
EVEN-ODD NUCLEI
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANES
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PSYCHOTROPIC DRUGS
RESONANCE
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques