Modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme a reductase

Journal Article · Fri Nov 30 23:00:00 EST 1984 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534735· OSTI ID:5558435

Georgopapadakou, N H; Dix, B A

A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.

• Research Organization: Squibb Institute for Medical Research, Princeton, NJ
• OSTI ID: 5558435
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 19:12; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BODY
CELL CULTURES
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
FIBROBLASTS
FUNGI
MICROORGANISMS
ORGANS
OXIDOREDUCTASES
PLANTS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOMETRIC ANALYSIS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SKIN
SOMATIC CELLS
YEASTS