Renal protein synthesis in diabetes mellitus: effects of insulin and insulin-like growth factor I

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · American Journal of Kidney Diseases; (United States)

DOI:https://doi.org/10.1016/S0272-6386(12)80345-3· OSTI ID:5554522

Barac-Nieto, M; Lui, S M; Spitzer, A ^[1]

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1. Department of Pediatrics, Albert Einstein College of Medicine, Bronx, NY (USA)

Is increased synthesis of proteins responsible for the hypertrophy of kidney cells in diabetes mellitus Does the lack of insulin, and/or the effect of insulin-like growth factor I (IGFI) on renal tubule protein synthesis play a role in diabetic renal hypertrophy To answer these questions, we determined the rates of 3H-valine incorporation into tubule proteins and the valine-tRNA specific activity, in the presence or absence of insulin and/or IGFI, in proximal tubule suspension isolated from kidneys of streptozotocin diabetic and control rats. The rate of protein synthesis increased, while the stimulatory effects of insulin and IGFI on tubule protein synthesis were reduced, early (96 hours) after induction of experimental diabetes. Thus, hypertrophy of the kidneys in experimental diabetes mellitus is associated with increases in protein synthesis, rather than with decreases in protein degradation. Factor(s) other than the lack of insulin, or the effects of IGFI, must be responsible for the high rate of protein synthesis present in the hypertrophying tubules of diabetic rats.

• OSTI ID: 5554522
• Journal Information: American Journal of Kidney Diseases; (United States), Journal Name: American Journal of Kidney Diseases; (United States) Vol. 17:6; ISSN 0272-6386; ISSN AJKDD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
GROWTH FACTORS
HORMONES
HYDROGEN COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
MAMMALS
METABOLIC DISEASES
MITOGENS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PEPTIDE HORMONES
PROTEINS
RATS
REACTION KINETICS
RNA
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRANSFER RNA
TRITIUM COMPOUNDS
TUBULES
VALINE
VERTEBRATES