O/sup 6/-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5554297
O/sup 6/-Methylguanine-DNA methyltransferase is induced in Escherichia coli during growth in low levels of N-methyl-N'-nitro-N-nitrosoguanidine. We have developed a sensitive assay for quantitating low levels of this activity with a synthetic DNA substrate containing /sup 3/H-labeled O/sup 6/-methylguanine as the only modified base. Although both wild-type and adaptation-deficient (ada) mutants of E. coli contained low but comparable numbers (from 13 to 60) of the enzyme molecules per cell, adaptation treatment caused a significant increase of the enzyme in the wild type but not in the ada mutants, suggesting that the ada mutation is in a regulatory locus and not in the structural gene for the methyltransferase.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5554297
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 152:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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