Measurement and chemical characterization of vapor-phase mutagens in diesel exhaust. Final report

Technical Report · Mon Mar 01 04:00:00 EST 1993

OSTI ID:5553551

Hsieh, D P.H.; Kado, N Y; Okamoto, R; Kuzmicky, P A; Rathbun, C

Methods for the collection, extraction, fractionation, and concentration of vapor-phase compounds from the undiluted exhaust of a medium heavy-duty diesel truck were developed and integrated with chemical analysis and a sensitive mutagenicity bioassay. A sampling system composed of a Teflon filter, polyurethane foam (PUF) and XAD-4 (XAD) adsorbents, in series, was used to directly trap PM and VM in the diesel truck exhaust. The VM on the PUF and XAD adsorbents were extracted with supercritical carbon dioxide (s-CO2) into concentrated methanol fractions which were directly analyzed by a specially modified Ames Salmonella mutagenicity assay and gas chromatograph interfaced to a quadrupole mass spectrometer (GC-MS). The PM-associated mutagens were extracted with organic solvents and assayed for mutagenicity.

• Research Organization: California Univ., Davis, CA (United States). Dept. of Environmental Toxicology
• OSTI ID: 5553551
• Report Number(s): PB-94-109907/XAB; CNN: ARB-A032-095
• Country of Publication: United States
• Language: English

Related Subjects

33 ADVANCED PROPULSION SYSTEMS
330700* -- Advanced Propulsion Systems-- Emission Control
ADSORBENTS
AIR POLLUTION MONITORING
ALCOHOLS
ALKANES
AROMATICS
BACTERIA
BENZENE
BIOASSAY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
DIESEL ENGINES
ENGINES
EXHAUST SYSTEMS
EXTRACTION
FLUORINATED ALIPHATIC HYDROCARBONS
FRACTIONATION
GAS CHROMATOGRAPHY
HALOGENATED ALIPHATIC HYDROCARBONS
HEAT ENGINES
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
INTERNAL COMBUSTION ENGINES
MASS SPECTROSCOPY
MATERIALS
MEASURING INSTRUMENTS
METHANOL
MICROORGANISMS
MONITORING
MUTAGENS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC POLYMERS
ORGANIC SOLVENTS
OXIDES
OXYGEN COMPOUNDS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLY
POLYAMIDES
POLYETHYLENES
POLYMERS
POLYTETRAFLUOROETHYLENE
POLYURETHANES
SALMONELLA
SAMPLING
SEPARATION PROCESSES
SOLVENTS
SPECTROMETERS
SPECTROSCOPY
STATISTICAL DATA
SYNTHETIC MATERIALS
TEFLON