Synthesis of gangliosides by cultured oligodendrocytes

Journal Article · Thu Jan 01 00:00:00 EST 1981 · J. Neurosci. Res.; (United States)

DOI:https://doi.org/10.1002/jnr.490060311· OSTI ID:5551023

Mack, S R; Szuchet, S; Dawson, G

Gangliosides are enriched in the nervous system compared to other tissues. The synthesis of gangliosides by monolayer cultures of isolated oligodendrocytes has not previously been investigated. Cells were labeled with (3H) galactose at preselected times and gangliosides isolated by phase partition, purified, and identified by chromatography. Cultured oligodendrocytes showed selectivity in their synthesis of gangliosides, which was expressed in the type of ganglioside synthesized as well as in the change of incorporation over time in culture. For the first ten days, there was very little incorporation of (3H) galactose in gangliosides, but this was followed by a stimulation of uptake for GM3, GM1/GD3, and GD1 gangliosides, reaching a maximum after approximately 25-30 days in vitro. There was little incorporation into GM2 or trisialogangliosides throughout the life of the cultures. Since oligodendrocytes synthesize extensive membranes during this period, one may speculate that the de novo-synthesized gangliosides are used for membranes.

• Research Organization: Hearst Laboratory of Neuroscience, Brain Research Institute, University of Chicago
• OSTI ID: 5551023
• Journal Information: J. Neurosci. Res.; (United States), Vol. 6:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GANGLIOSIDES
BIOSYNTHESIS
TRITIUM COMPOUNDS
TRACER TECHNIQUES
CELL CULTURES
GALACTOSE
NERVE CELLS
ALDEHYDES
ANIMAL CELLS
CARBOHYDRATES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques