Comparative examination of deep subsurface microorganisms at geographically and climatically diverse locations on DOE lands

Technical Report · Tue Jul 30 04:00:00 EDT 1991

OSTI ID:5548026

Balkwill, D L; Reeves, R H

This is the final technical report for a project associated with the DOE Transitional Phase Deep Microbiology Program, a subprogram of the DOE Subsurface Science Program. There are three major activities described for this funding period. The first major activity was program coordination (planning and organization) leading up to field sampling at the Idaho National Engineering Laboratory and the Hanford Reservation, with preliminary analysis of the samples. Next, aerobic, chemoheterotrophic microorganisms were isolated, counted and partially characterized. Finally, a phylogenetic study was performed using 16S rRNA sequences and Restriction Fragment Length Polymorphism (RFLP) analysis. With this data, phylogenetic relationships were determined between the isolated organisms and microbial taxa were established. 13 refs., 1 tab. (MHB)

• Research Organization: Florida State Univ., Tallahassee, FL (United States). Dept. of Biological Science
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-89ER60774
• OSTI ID: 5548026
• Report Number(s): DOE/ER/60774-2; ON: DE91016485
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BIOLOGY
DOCUMENT TYPES
ENVIRONMENTAL MATERIALS
GENETICS
GROUND WATER
HANFORD RESERVATION
HYDROGEN COMPOUNDS
HYDROLOGY
IDAHO NATIONAL ENGINEERING LABORATORY
MATERIALS
MICROORGANISMS
MORPHOLOGY
NATIONAL ORGANIZATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROGRESS REPORT
RFLPS
RIBOSOMAL RNA
RNA
SITE CHARACTERIZATION
SITE SURVEYS
SPECIES DIVERSITY
SUBSURFACE ENVIRONMENTS
TAXONOMY
US DOE
US ERDA
US ORGANIZATIONS
WATER