Autoradiographic localization of binding sites for galanin and VIP in small intestine

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Peptides (Fayetteville, New York); (USA)

DOI:https://doi.org/10.1016/0196-9781(89)90036-3· OSTI ID:5547414

King, S C; Slater, P; Turnberg, L A ^[1]

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1. Univ. of Manchester (England)

The distribution of the binding sites for ({sup 125}I)galanin and ({sup 125}I)iodotyrosyl VIP in sections of small intestine from rat, rabbit, guinea pig and man was investigated using in vitro receptor autoradiography. The specific binding sites for ({sup 125}I)galanin were most concentrated in the myenteric plexus and longitudinal muscle layers. The distribution of ({sup 125}I)VIP binding was similar, with the highest level of binding in the myenteric plexus. Some VIP binding was also seen in the mucosa. The abundance of binding sites for both peptides in the myenteric plexus suggests that this is the anatomical site of the functional antagonism between these endogenous peptides in the mammalian intestine.

• OSTI ID: 5547414
• Journal Information: Peptides (Fayetteville, New York); (USA), Journal Name: Peptides (Fayetteville, New York); (USA) Vol. 10:2; ISSN PPTDD; ISSN 0196-9781
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GUINEA PIGS
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PRIMATES
PROTEINS
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SMALL INTESTINE
VERTEBRATES