Regeneration of bovine and octopus opsins in situ with natural and artificial retinals

Journal Article · Mon Mar 20 23:00:00 EST 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00432a055· OSTI ID:5547144

Koutalos, Y; Ebrey, T G; Tsuda, M; Odashima, K; Lien, T; Park, M H; Shimizu, N; Derguini, F; Nakanishi, K; Gilson, H R; Honig, B ^[1]

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1. Univ. of Illinois, Urbana-Champaign (USA)

The authors consider the problem of color regulation in visual pigments for both bovine rhodopsin and octopus rhodopsin. Both pigments have 11-cis-retinal as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins were regenerated with natural and artificial chromophores. Both bovine and octopus opsins were regenerated with the 9-cis- and 11-cis-retinal isomers, but the octopus opsin was additionally regenerated with the 13-cis and all-trans isomers. Titration of the octopus opsin with 11-cis-retinal gave an extinction coefficient for octopus rhodopsin of 27,000 {plus minus} 3,000 M{sup {minus}1} cm{sup {minus}1} at 475 nm. The absorption maxima of bovine artificial pigments formed by regenerating opsin with the 11-cis dihydro series of chromophores support a color regulation model for bovine rhodopsin in which the chromophore-binding site of the protein has two negative charges: one directly hydrogen bonded to the Schiff base nitrogen and another near carbon-13. Formation of octopus artificial pigments with both all-trans and 11-cis dihydro chromophores leads to a similar model for octopus rhodopsin and metarhodopsin: there are two negative charges in the chromophore-binding site, one directly hydrogen bonded to the Schiff base nitrogen and a second near carbon-13. The interaction of this second charge with the chromophore in octopus rhodopsin is weaker than in bovine, while in metarhodopsin it is as strong as in bovine.

• OSTI ID: 5547144
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:6; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
ANIMALS
AQUATIC ORGANISMS
CARBON 13
CARBON ISOTOPES
CATTLE
CHEMICAL BONDS
CHROMATOGRAPHY
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
MOLLUSCS
NUCLEI
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
RHODOPSIN
RUMINANTS
SEPARATION PROCESSES
SPECTRA
STABLE ISOTOPES
VERTEBRATES
VISION
VITAMIN A
VITAMINS