Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.19.7211· OSTI ID:5546989

Chang, C; Kokontis, J; Liao, S ^[1]

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1. Univ. of Chicago, IL (USA)

Structural analysis of cDNAs for human and rat androgen receptors (ARs) indicates that the amino-terminal regions of ARs are rich in oligo- and poly(amino acid) motifs as in some homeotic genes. The human AR has a long stretch of repeated glycines, whereas rat AR has a long stretch of glutamines. There is a considerable sequence similarity among ARs and the receptors for glucocorticoids, progestins, and mineralocorticoids within the steroid-binding domains. The cysteine-rich DNA-binding domains are well conserved. Translation of mRNA transcribed from AR cDNAs yielded 94- and 76-kDa proteins and smaller forms that bind to DNA and have high affinity toward androgens. These rat or human ARs were recognized by human autoantibodies to natural Ars. Molecular hybridization studies, using AR cDNAs as probes, indicated that the ventral prostate and other male accessory organs are rich in AR mRNA and that the production of AR mRNA in the target organs may be autoregulated by androgens.

• OSTI ID: 5546989
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:19; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACID SEQUENCE
AMINO ACIDS
ANDROGENS
ANDROSTANES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CORTICOSTEROIDS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GENES
GLUCOCORTICOIDS
HORMONES
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
KETONES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
METHIONINE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PREGNANES
PRIMATES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RECOMBINANT DNA
RNA
RODENTS
STEROID HORMONES
STEROIDS
SULFUR 35
SULFUR ISOTOPES
THIOLS
TRITIUM COMPOUNDS
VERTEBRATES