Labeling of proteins with prechelated radionuclides

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:5545447

Misellati, K M

A number of ligands, some of which were bifunctional, containing pyrrole, phenol, or pyridine coordinating groups were synthesized. The radiochemistry of the complexes of the ligands with several radionuclides ({sup 105}Rh, {sup 109}Pd, {sup 99m}Tc, {sup 64}Cu,{sup 57}Cu, {sup 198}Au, {sup 111}In) was studied. Complexation of most of these radionuclides was accomplished by simple mixing of reagents without a need for any rigorous conditions, but rhodium complexes were prepared by refluxing in alcohol. Complex yields were determined by chromatography, adsorption on magnesium oxide or by solvent extraction. Conjugation of all the bifunctional complexes with human {gamma}-globulin was attempted by activating with thiophosgene and incubating at room temperature with the protein. Gel filtration chromatography was the main technique used for determining the conjugation yield. The stability of the coupled protein was studied by challenge experiments using EDTA and cyclam. High and stable conjugation yields were obtained with bifunctional complexes of most of the above radionuclides. The use of prechelated radionuclides for labeling proteins offer many advantages, most importantly, it reduces the nonspecific labeling and eliminates the exposure of the protein to too many harsh conditions normally needed for complexation and conjugation which frequently result in excessive denaturation of the labeled biomacromolecules. This technique provides an easy and efficient approach to the radionuclide labeling of other biologically active macromolecules such as antibody fragments.

• Research Organization: Missouri Univ., Columbia, MO (United States)
• OSTI ID: 5545447
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CHEMISTRY
CHROMATOGRAPHY
COMPLEXES
COPPER 57
COPPER 64
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GEL PERMEATION CHROMATOGRAPHY
GLOBULINS
GOLD 198
GOLD ISOTOPES
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LIGANDS
MAMMALS
MAN
MILLISEC LIVING RADIOISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PALLADIUM 109
PALLADIUM ISOTOPES
PRIMATES
PROTEINS
RADIOCHEMISTRY
RADIOISOTOPES
RHODIUM 105
RHODIUM ISOTOPES
SECONDS LIVING RADIOISO
SEPARATION PROCESSES
TECHNETIUM 99
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOI