Antithrombin III-beta associates more readily than antithrombin III-alpha with uninjured and de-endothelialized aortic wall in vitro and in vivo

Journal Article · Wed May 01 04:00:00 EDT 1991 · Arterioscleros Thrombos; (USA)

DOI:https://doi.org/10.1161/01.ATV.11.3.530· OSTI ID:5545390

Witmer, M R; Hatton, M W ^[1]

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1. Univ. Health Sciences Centre, Hamilton, ON, (Canada)

The properties of two isoforms, alpha and beta, of rabbit antithrombin III (ATIII) were compared in the presence of undamaged or de-endothelialized rabbit aortic wall. Similar quantities of ATIII-alpha and ATIII-beta bound to and rapidly saturated the endothelium in vitro, but the rate of transendothelial passage of ATIII-beta exceeded that of ATIII-alpha by 22%. Furthermore, ATIII-beta was adsorbed approximately twice as rapidly as ATIII-alpha by the subendothelium of the de-endothelialized aorta. Binding of both isoforms was decreased (ATIII-beta more than ATIII-alpha) by pretreating the subendothelial surface with heparitinase. Also, subendothelium-bound ATIII-beta was desorbed more readily than bound ATIII-alpha by thrombin. In vivo, the rate of uptake of iodine-131-labeled ATIII-beta from the circulation by the aortic wall and the major organs was 30-50% faster than that of iodine-125-labeled ATIII-alpha. In contrast, the uptake of {sup 131}I-ATIII-beta by the de-endothelialized aorta in vivo was three times faster than that of {sup 125}I-ATIII-alpha. By these criteria, ATIII-beta is the more active of the two isoforms. We surmise that plasma and, consequently, vessel wall levels of ATIII-beta may be vital for controlling thrombogenic events caused by injury to the vascular wall.

• OSTI ID: 5545390
• Journal Information: Arterioscleros Thrombos; (USA), Journal Name: Arterioscleros Thrombos; (USA) Vol. 11:3; ISSN XZ045; ISSN 1049-8834
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PROTEINS
RABBITS
RADIOISOTOPES
REACTION KINETICS
SERINE PROTEINASES
SORPTION
THROMBIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES