The effect of retinol on the hyperthermal response of normal tissue in vivo
The effect of prior administration of retinol, a membrane labilizer, on the in vivo hyperthermal response of lysosomes was investigated in the mouse spleen using a quantitative histochemical assay for the lysosomal enzyme acid phosphatase. A dose of retinol which had no effect when given alone enhanced the thermal response of the lysosome, causing an increase in lysosomal membrane permeability. In contrast, the same dose of retinol had no effect on the gross hyperthermal response of mouse intestine; a tissue which is relatively susceptible to hyperthermia. Thermal damage to intestine was assayed directly by crypt loss 1 day after treatment or assessed as thermal enhancement of X-ray damage by counting crypt microcolonies 4 days after a combined heat and X-ray treatment. Thus, although the hyperthermal response of the lysosome could be enhanced by the administration of retinol, thermal damage at a gross tissue level appeared to be unaffected, suggesting that lysosomal membrane injury is unlikely to be a primary event in hyperthermal cell killing.
- Research Organization:
- MRC Cyclotron Unit, Hammersmith Hospital, London, England
- OSTI ID:
- 5544816
- Journal Information:
- Radiat. Res.; (United States), Vol. 95:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SMALL INTESTINE
BIOLOGICAL RADIATION EFFECTS
TEMPERATURE EFFECTS
VITAMIN A
BIOLOGICAL EFFECTS
ACID PHOSPHATASE
LYSOSOMES
MICE
X RADIATION
ANIMALS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GASTROINTESTINAL TRACT
HYDROLASES
INTESTINES
IONIZING RADIATIONS
MAMMALS
ORGANOIDS
ORGANS
PHOSPHATASES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
VITAMINS
560205* - Thermal Effects- Vertebrates- (-1987)
560152 - Radiation Effects on Animals- Animals