Clonal deletion of self-reactive T cells in irradiation bone marrow chimeras and neonatally tolerant mice. Evidence for intercellular transfer of Mlsa

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Journal of Experimental Medicine; (USA)

DOI:https://doi.org/10.1084/jem.170.2.595· OSTI ID:5543127

Speiser, D E; Schneider, R; Hengartner, H; MacDonald, H R; Zinkernagel, R M ^[1]

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1. Univ. Hospital, Zuerich (Switzerland)

Tolerance to Mlsa has been shown to be associated with clonal deletion of cells carrying TCR beta chain variable regions V beta 6 or V beta 8.1 in mice possessing I-E antigens. To evaluate the rules of tolerance induction to Mlsa we prepared irradiation bone marrow chimeras expressing Mlsa or Mlsb and I-E by different cell types. Deletion of V beta 6+, Mlsa-reactive T cells required the presence of Mlsa and I-E products either on bone marrow-derived cells or on irradiated recipient cells. Tolerance was induced when Mlsa and I-E were expressed by distinct cells of the chimera. Also neonatally tolerized mice exhibited depletion of V beta 6+ cells after injection of I-E- Mlsa spleen cells (DBA/1) into newborn I-E+ Mlsb mice (BALB/c x B10.G)F1. These results suggest that the product of the Mlsa locus is soluble and/or may be transferred from cell to cell and bound to I-E antigens. The chimera experiments also showed that tolerance to Mlsa is H-2 allele independent, i.e., is apparently unrestricted. Differentiation of chimeric (H-2d/Mlsa x H-2q/Mlsb)F1 stem cells in either an H-2d or an H-2q thymus revealed that tolerance assessed by absence of V beta 6+ T cells is not dependent on the thymically determined restriction specificity of T cells.

• OSTI ID: 5543127
• Journal Information: Journal of Experimental Medicine; (USA), Journal Name: Journal of Experimental Medicine; (USA) Vol. 170:2; ISSN 0022-1007; ISSN JEMEA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL DIFFERENTIATION
CHIMERAS
CONNECTIVE TISSUE CELLS
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MOSAICISM
NEONATES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION CHIMERAS
RECEPTORS
RODENTS
SOMATIC CELLS
SPECIFICITY
SPLEEN CELLS
STEM CELLS
THYMUS
TISSUES
TOLERANCE
VERTEBRATES