Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects
Northern blot hybridizations showed that mRNA{sub f} is female-specific, and that both mRNAs are liver-specific and expressed at high levels only during the fall and winter in the wild. When warm-acclimated flounder (18C) were acclimated over 2 days to low temperature (4 C) and short photoperiod in July and October, high levels of AF mRNA accumulated within 4-10 days. Low levels were found in fish at high temperature at any time, on long photoperiod after acclimation to low temperature in July, or continuously at 4 C from March until September. A temperature increase in January resulted in a dramatic decrease in the levels of AF mRNA within 5 days. Beta actin mRNA, mRNA{sub f} and most mRNAs translated in a reticulocyte lysate did not change in abundance after temperature increases or decreases. Synthesis of AF mRNA in liver slices was investigated by Northern blot hybridization and hybridization of {sup 32}P-labeled RNA to AF cDNA. Liver slices from cold-acclimated January fish expressed high levels of AF mRNA initially at 4 C and lower levels at 18 C. AF mRNA expression was turned off in tissue from warm-acclimated fish but was progressively induced after long 4 C incubations in vitro in livers from fall fish but not from spring fish. Temperature and photoperiod act either as zeitgebers, which set the phase of an endogenous rhythm, or as more direct signals that stimulate a transient accumulation of AF mRNA.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD (USA)
- OSTI ID:
- 5542692
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
FISHES
TEMPERATURE EFFECTS
MESSENGER-RNA
SPECIFICITY
DNA HYBRIDIZATION
GENE REGULATION
LIVER
PHOSPHORUS 32
SEASONAL VARIATIONS
SEX DEPENDENCE
TRACER TECHNIQUES
TRANSCRIPTION
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
GLANDS
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
VARIATIONS
VERTEBRATES
560200* - Thermal Effects
550201 - Biochemistry- Tracer Techniques