Transformation by complementation of an adenine auxotroph of the lignin-degrading basidiomycete Phanerochaete chrysosporium

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Applied and Environmental Microbiology; (United States)

OSTI ID:5534338

Alic, M; Kornegay, J R; Pribnow, D; Gold, M H ^[1]

---

1. Oregon Graduate Center, Beaverton (United States)

Swollen basiodiospores of an adenine auxotroph of Phanerochaete chrysosporium were protoplasted with Novozyme 234 and transformed to prototrophy by using a plasmid containing the gene for an adenine biosynthetic enzyme from Schizophyllum commune. Transformation frequencies of 100 transformants per {mu}g of DNA were obtained. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Analysis of conidia and basiodiospores from transformants demonstrated that the transforming character was mitotically and meiotically stable on both selective and nonselective media. Genetic crosses between double mutants transformed for adenine prototrophy and other auxotrophic strains yielded Ade{sup {minus}} progeny, which indicated that integration occurred at a site(s) other than the resident adenine biosynthetic gene.

• DOE Contract Number: FG06-86ER13550
• OSTI ID: 5534338
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 55:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIODEGRADATION
BIOTECHNOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CELL DIVISION
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ENZYMES
FUNGI
GENE RECOMBINATION
GENES
GENETIC ENGINEERING
LIGNIN
MEIOSIS
MITOSIS
MOLECULAR BIOLOGY
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PLANTS
PLASMIDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES