Close relationship between modulation of serum-induced stimulation of DNA synthesis and changes in gap-junctional intercellular communication in quiescent 3T3-L1 cells caused by cyclic AMP and the tumor-promoting phorbol ester TPA
- Hiroshima Univ. School of Dentistry (Japan)
Involvement of gap-junctional intercellular communication in the stimulation of growth was investigated in quiescent 3T3-L1 cells. When the cells in monolayer were growth-arrested by culture in a low concentration of calf serum, addition of dibutyryl cyclic AMP enhanced dye-coupling and suppressed the enhancement of DNA synthesis, induced by calf serum, in quiescent cells. 12-O-Tetradecanoylphorbol-13-acetate (TPA) suppressed dye-coupling in quiescent cells and enhanced DNA synthesis in both quiescent and serum-treated cells. When about 5000 cells were cultured in contact to form a colony, growth arrest of the cells was observed in the central region of such colonies rather than in the peripheral region, but addition of calf serum induced DNA synthesis in the cells in both the peripheral and central regions of the colonies. Addition of TPA enhanced serum-induced DNA synthesis in the cells in the central region of colonies rather than in the peripheral region. These results suggest that the ability of quiescent cells to escape from growth arrest is inversely correlated to the extent of gap-junctional intercellular communication.
- OSTI ID:
- 5534046
- Journal Information:
- Experimental Cell Research; (United States), Vol. 185:2; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
Similar Records
Changes of intracellular and externally bound cations accompanying serum stimulation of mouse BALB/c 3T3 cells
Changes of intracellular and externally bound cations accompanying serum stimulation of mouse BALB/c 3T3 cells
Related Subjects
AMP
BIOLOGICAL EFFECTS
DNA REPLICATION
MOLECULAR BIOLOGY
PHORBOL ESTERS
BUDR
CELL PROLIFERATION
CYTOLOGICAL TECHNIQUES
FIBROBLASTS
TUMOR PROMOTERS
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BROMOURACILS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DRUGS
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROMOTERS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
URACILS
550300* - Cytology