Indirect assays for deoxyhypusine hydroxylase using dual-label ratio changes and oxidative release of radioactivity

Journal Article · Thu May 01 00:00:00 EDT 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90044-8· OSTI ID:5532195

Abbruzzese, A; Park, M H; Folk, J E

Two procedures for rapid assay of deoxyhypusine hydroxylase activity are described. One of these assays measures changes in the /sup 3/H:/sup 14/C ratio of dual-labeled protein that results from the release of tritium from a specific position in the side chain of the /sup 3/H, /sup 14/C-labeled constituent amino acid deoxyhypusine upon its conversion to (/sup 3/H, /sup 14/C)hypusine. The other assay relies upon release of radioactivity from product protein by periodate oxidation of the radiolabeled side chain of component hypusine. The good correspondence of each of these assays with the ion exchange chromatographic method which measures hypusine and deoxyhypusine in acid hydrolysates of protein indicates that each provides a valid means of determining deoxyhypusine hydroxylase activity.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5532195
• Journal Information: Anal. Biochem.; (United States), Vol. 154:2
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
HYDROXYLASES
RADIO-RELEASE ANALYSIS
AMINO ACIDS
CARBON 14 COMPOUNDS
DOUBLE LABELLING
ENZYME ACTIVITY
ION EXCHANGE CHROMATOGRAPHY
OXIDATION
PERIODIC ACID
PROTEINS
TRITIUM COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
ENZYMES
HYDROGEN COMPOUNDS
INORGANIC ACIDS
LABELLED COMPOUNDS
LABELLING
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
400700* - Radiochemistry & Nuclear Chemistry
550201 - Biochemistry- Tracer Techniques