Studies on transposable elements in yeast. I. ROAM mutations causing increased expression of yeast genes: their activation by signals directed toward conjugation functions and their formation by insertion of Tyl repetitive elements

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Cold Spring Harbor Symp. Quant. Biol.; (United States)

DOI:https://doi.org/10.1101/SQB.1981.045.01.077· OSTI ID:5530167

Errede, B; Cardillo, T S; Wever, G; Sherman, F

Mechanisms available to eukaryotic organisms for the coordinate regulation of gene expression are being examined by genetic and biochemical characterization of an unusual mutation, CYC7-H2, which causes over-production of iso-2-cytochrome c in the yeast Saccharomyces cerevisiae. The CYC7-H2 mutation causes overproduction in haploid strains but only a 1- to 40-fold overproduction in MATa/MAT..cap alpha.. diploid strains. This regulation of overproduction has been characterized as a response to signals controlling conjugation in yeast. Furthermore, the abnormal controlling region has been identified as an insertion of a transposable and reiterated Ty1 element adjacent to the structural gene. Therefore, we suggest that Ty1 elements or portions of Ty1 elements occur adjacent to some of the genes required for conjugation and that they normally function to control expression of this process. The suggested role of reiterated sequences may represent a general mechanism of coordinate regulation in eukaryotes. The CYC7-H2 mutation is closely related to other regulatory mutations occurring at the cargA, cargB and DUR1,2 loci. Similar to the CYC7-H2 mutation, the mutations designated cargA/sup +/O/sup h/, cargB/sup +/O/sup h/, and durO/sup h/ cause constitutive production of their respective gene products at much lower levels of MATa/MAT..cap alpha.. diploid strains than in the corresponding haploid strains. A consistent relationship between conjugation competence and the level of overproduction in all four mutants has been established. Observations characterizing the regulation of overproduction in the CYC7-H2 mutant are presented with the additional and parallel observations for the O/sup h/ mutants. Together these results provide a demonstration of the specificity and equivalence of regulatory control exhibited by ROAM mutants.

• Research Organization: Univ. of Rochester, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 5530167
• Journal Information: Cold Spring Harbor Symp. Quant. Biol.; (United States), Journal Name: Cold Spring Harbor Symp. Quant. Biol.; (United States) Vol. 45; ISSN CSHSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
CYTOCHROMES
ENZYME ACTIVITY
FUNGI
GENE MUTATIONS
GENES
GENETIC EFFECTS
MATING
MICROORGANISMS
MUTANTS
MUTATIONS
PIGMENTS
PLANTS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
YEASTS