Growth of a radiation-transformed clone of C3H 1OT1/2 cells into melanin-producing colonies

Journal Article · Mon Dec 31 23:00:00 EST 1984 · Scanning Electron Microsc.; (United States)

OSTI ID:5528813

Szekely, J G; Raaphorst, G P; Lobreau, A U; Azzam, E I; Vadasz, J A

When R25, a radiation-transformed clone of C3H 1OT1/2 cells, was plated in soft agarose, a fraction of the colonies became pigmented. The morphologies of the white and dark colonies and their cells were compared by optical, transmission and scanning electron microscopy. The transformed R25 cells had apparently differentiated into melanin-producing cells in the soft agarose, with the white colonies containing actively growing cells having only a few melanosomes, and the dark colonies being made up of stationary-phase cells filled with electronopaque melanosomes. Exposure of the R25 cells to 4.0 Gy of X-rays decreased the percentage of dark colonies, while exposure to 1% DMSO had no effect.

• Research Organization: Atomic Energy of Canada Limited Research Co., Pinawa, Manitoba
• OSTI ID: 5528813
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States); ISSN SEMYB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CELL CULTURES
CELL DIVISION
CLONE CELLS
GROWTH
HYDROXY COMPOUNDS
MAMMALS
MELANIN
MICE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
RADIATION EFFECTS
RODENTS
SYNTHESIS
VERTEBRATES