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Assay of ATP-sulfurylase

Journal Article · · Anal. Biochem.; (United States)

An assay method for ATP-sulfurylase is described, based on the incorporation of /sup 35/SO/sub 4//sup 2 -/ into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20/sup 0/C during the assay, the reaction time should not exceed 10 min.

Research Organization:
Medical College of Georgia, Augusta
OSTI ID:
5528591
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 154:1; ISSN ANBCA
Country of Publication:
United States
Language:
English