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Title: Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva

Abstract

Biochemical and molecular techniques have been used to study the formation and recovery of the developmentally arrested, non-feeding dauer stage of the nematode Caenorhabditis elegans. While investigating developmental transitions in energy metabolism, a major metabolite isolated from perchloric acid extracts has been identified as a modified uridine nucleotide. The compound was isolated by gel filtration and ion-exchange chromatography and its structure was determined by {sup 1}H NMR and {sup 13}C NMR spectroscopy. This compound is the most abundant metabolite detected in {sup 31}PMR spectra of perchloric acid extracts from growing larvae. In the absence of phosphoarginine or phosphocreatine, this modified nucleotide may have an important function in the nematode's energy metabolism, and it may also be found in several other invertebrates. During recovery from the dauer stage, metabolic activation is accompanied by a decrease in intracellular pH (pH{sub i}). Although metabolic activation has been associated with an alkaline pH{sub i} shift in other organisms, in vivo {sup 31}P NMR analysis of recovering dauer larvae shows a pH{sub i} decrease from {approximately}7.3 to {approximately}6.3 within 3 hr after the animals encounter food. This shift occurs before feeding begins, and coincides with, or soon follows, the development commitment to recover from themore » dauer stage, suggesting that control of pH{sub i} may be important in the regulation of larval development in nematodes. A library enriched for sequences expressed specifically during the L2d (predauer) stage was made by selecting plaques from a genomic lambda library that hybridized to subtracted L2d cDNA probes. Ultimately, three clones that were shown to hybridize only to L2d RNA were selected.« less

Authors:
Publication Date:
Research Org.:
Missouri Univ., Columbia, MO (USA)
OSTI Identifier:
5526544
Alternate Identifier(s):
OSTI ID: 5526544
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; METABOLITES; MOLECULAR BIOLOGY; NEMATODES; METABOLISM; BIOCHEMISTRY; CARBON 13; CHEMICAL SHIFT; CHROMATOGRAPHY; DNA HYBRIDIZATION; GENES; LARVAE; NMR SPECTRA; NUCLEOTIDES; PHOSPHORUS 31; PROTONS; TRACER TECHNIQUES; ASCHELMINTHES; BARYONS; CARBON ISOTOPES; CHEMISTRY; ELEMENTARY PARTICLES; EVEN-ODD NUCLEI; FERMIONS; HADRONS; HELMINTHS; HYBRIDIZATION; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEONS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; SEPARATION PROCESSES; SPECTRA; STABLE ISOTOPES 550501* -- Metabolism-- Tracer Techniques; 550201 -- Biochemistry-- Tracer Techniques

Citation Formats

Wadsworth, W.G.. Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva. United States: N. p., 1989. Web.
Wadsworth, W.G.. Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva. United States.
Wadsworth, W.G.. Sun . "Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva". United States. doi:.
@article{osti_5526544,
title = {Biochemistry and molecular biology of the Caenorhabditis elegans dauer larva},
author = {Wadsworth, W.G.},
abstractNote = {Biochemical and molecular techniques have been used to study the formation and recovery of the developmentally arrested, non-feeding dauer stage of the nematode Caenorhabditis elegans. While investigating developmental transitions in energy metabolism, a major metabolite isolated from perchloric acid extracts has been identified as a modified uridine nucleotide. The compound was isolated by gel filtration and ion-exchange chromatography and its structure was determined by {sup 1}H NMR and {sup 13}C NMR spectroscopy. This compound is the most abundant metabolite detected in {sup 31}PMR spectra of perchloric acid extracts from growing larvae. In the absence of phosphoarginine or phosphocreatine, this modified nucleotide may have an important function in the nematode's energy metabolism, and it may also be found in several other invertebrates. During recovery from the dauer stage, metabolic activation is accompanied by a decrease in intracellular pH (pH{sub i}). Although metabolic activation has been associated with an alkaline pH{sub i} shift in other organisms, in vivo {sup 31}P NMR analysis of recovering dauer larvae shows a pH{sub i} decrease from {approximately}7.3 to {approximately}6.3 within 3 hr after the animals encounter food. This shift occurs before feeding begins, and coincides with, or soon follows, the development commitment to recover from the dauer stage, suggesting that control of pH{sub i} may be important in the regulation of larval development in nematodes. A library enriched for sequences expressed specifically during the L2d (predauer) stage was made by selecting plaques from a genomic lambda library that hybridized to subtracted L2d cDNA probes. Ultimately, three clones that were shown to hybridize only to L2d RNA were selected.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}

Miscellaneous:
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