The mutation studies of mutagen-sensitive and DNA repair mutants of Chinese hamster fibroblasts
We have previously reported the isolation and partial characterization of DNA repair and/or mutagen-sensitive mutant Chinese hamster cell strains. Here we present the results of a detailed study of the ultraviolet light (UV)-induced mutability of one of these strains, UVs-7, and provide preliminary mutability data on two additional lines, UVr-23 and UVs-40. UVs-7 in extremely deficient in unscheduled DNA synthesis (UDS) but only slightly more sensitive to UV than the parental line. When examined for the UV-inducibility of mutants resistant to ouabain, 6-thioguanine, or diphtheria toxin, UVs-7 was found to be hypermutable at all three loci as compared to the parental line. The degree of hypermutability was not the same for any two loci. UVs-40, a highly UV-sensitive strain, was also found to be hypermutable at the ouabain-resistant (ouar) locus. UVr-23, which is UV-resistant and more proficient at UDS than the parental line, appeared to exhibit a tendency toward hypomutability at both the ouabain(ouar) and 6-thioguanine--resistant (6TGr) loci. Further characterization of all these lines should aid in delineating mammalian mechanisms of DNA repair and mutagenesis.
- OSTI ID:
- 5525269
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 3:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
GENE MUTATIONS
BIOLOGICAL REPAIR
GENES
RADIOSENSITIVITY
CELL CULTURES
DNA
HAMSTERS
MUTAGENESIS
MUTANTS
OUABAIN
TOXINS
ULTRAVIOLET RADIATION
ANIMALS
ANTIGENS
BIOLOGICAL RECOVERY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ELECTROMAGNETIC RADIATION
GLYCOSIDES
MAMMALS
MATERIALS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
RODENTS
STROPHANTHINS
TOXIC MATERIALS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550400 - Genetics
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)