Effect of freezing on the coupling of VIP receptors to adenylate cyclase in rat liver membranes

Journal Article · Thu Dec 31 23:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90090-2· OSTI ID:5521094

Robberecht, P; Waelbroeck, M; De Neef, P; Camus, J C; Gourlet, P; Christophe, J

In fresh rat liver plasma membranes high affinity VIP receptors were specifically labelled with (/sup 125/I) helodermin and were well coupled to adenylate cyclase while low affinity VIP receptors were not. After freezing and thawing low affinity VIP receptors were also coupled to adenylate cyclase. This modification of adenylate cyclase activation was specific for the VIP response as freezing and thawing did not modify Gpp(NH)p, NaF and glucagon stimulations. 26 references, 3 figures, 1 table.

• Research Organization: Universit Libre de Bruxelles, Belgium
• OSTI ID: 5521094
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:5; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGY
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CRYOBIOLOGY
CYCLASES
DATA
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FREEZING
GLANDS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
LYASES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
THAWING
TRACER TECHNIQUES
VERTEBRATES