Neuropeptide Y administration acutely increases hypothalamic corticotropin-releasing factor immunoreactivity: lack of effect in other rat brain regions
The effect of acute central administration of Neuropeptide Y (NPY) to adult male rats on the brain content of corticotropin-releasing factor immunoreactivity (CRF-ir) was investigated. The brain regions studied included frontal cortex, hippocampus, medulla-pons, midbrain-thalamus, cerebellum, neurointermediate lobe of pituitary, median eminence and the remaining hypothalamus. CRF-ir was determined in each of these regions using radioimmunoassay specific for rat CRF. CRF-ir was found to be significantly increased in the major site of CRF localization in the brain, the hypothalamus, in NPY-treated rats as compared to vehicle-treated controls either 15 minutes (p<0.025) or 45 minutes (p<0.005) post-injection. This increase was localized to the median eminence (p<0.05 after 15 minutes, p<0.01 after 45 minutes). No statistically significant differences were noted in any of the other brain regions assessed. Plasma adrenocorticotropin levels were also found to increase following NPY treatment, an effect which became significant after 45 minutes (p<0.05). These data show that NPY can alter the content of hypothalamic CRF and may play a role in its regulation. 33 references, 4 figures.
- Research Organization:
- Univ. of Toronto, Ontario
- OSTI ID:
- 5520965
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:25; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DATA
EXPERIMENTAL DATA
FUNCTIONS
HYPOTHALAMUS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES