Enzyme-linked immunosorbent assay for leucine and methionine enkephalins

Journal Article · Thu Mar 31 23:00:00 EST 1983 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(83)90652-8· OSTI ID:5519836

Zamboni, G; Jones, C A; Hughes, J

An enzyme-linked immunosorbent assay for enkephalins was developed by coupling the peptides to a carrier molecule (bovine serum albumin) in order to allow the antibody-antigen reaction to take place in the solid phase. The assay was shown to be highly reproducible. Its sensitivity was 14 nmol/liter for leucine enkephalin and 27 nmol/liter for methionine enkephalin, which is similar to that obtained when the same antibodies were used in radioimmunoassay.

• Research Organization: Imperial Coll. of Science and Tech., London, UK
• OSTI ID: 5519836
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 130:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGEN-ANTIBODY REACTIONS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISORPTION
DRUGS
ENDORPHINS
ENKEPHALINS
ENZYMES
ISOTOPE APPLICATIONS
LEUCINE
LIPOTROPIC FACTORS
METHIONINE
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
QUALITATIVE CHEMICAL ANALYSIS
RADIOASSAY
RADIOIMMUNOASSAY
SENSITIVITY
SEPARATION PROCESSES
SORPTION
TRACER TECHNIQUES