Cloning and expression of full-length cDNA encoding human vitamin D receptor

Journal Article · Sun May 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.10.3294· OSTI ID:5517872

Baker, A R; McDonnell, D P; Hughes, M; Crisp, T M; Mangelsdorf, D J; Haussler, M R; Pike, J W; Shine, J; O'Malley, B W ^[1]

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1. California Biotechnology Inc., Mountain View (USA)

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3{prime} noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of {approx} 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

• OSTI ID: 5517872
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:10; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACID SEQUENCE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHOLECALCIFEROL
CLONING
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GASTROINTESTINAL TRACT
GLUCOCORTICOIDS
HORMONES
HYBRIDIZATION
HYDROXY COMPOUNDS
INTESTINES
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROGESTERONE
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RNA
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
THYROID HORMONES
VERTEBRATES
VITAMIN D
VITAMINS