The effect of BAY o 2752 on bile acid absorption and cholesterol esterification

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5516571

Harnett, K M

BAY o 2752 (N,N-(1,11-undecandiyl)bis(2,3-dihydro-2-methyl-1H-indole-1-carboxamide)) has been demonstrated to inhibit intestinal cholesterol absorption in rats. Studies were carried out on male Wistar rats to determine if this drug alters intestinal bile acid absorption or cholesterol esterification by acyl CoA: cholesterol acyltransferase (ACAT) or cholesterol ester hydrolase (CEH). BAY o 2752 did not affect intestinal absorption of taurocholic acid (TC) from ileal segments perfused in vivo with a tragacanth suspension in phosphate buffer containing NaCl, TC, and 24-{sup 14}C-TC as determined by the excretory rate of radioactivity in bile. BAY o 2752 also did not affect the uptake of TC into ileal everted sacs incubated in stirred, gassed Krebs-Ringer bicarbonate buffer with 1 mM TC, 24-{sup 14}C-TC and {sup 3}H-inulin. BAY o 2752 also did not bind TC; TG, in a filtrate of the above solutions remained at 92-98% of control.

• Research Organization: Boston Univ., MA (USA)
• OSTI ID: 5516571
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMIDES
BIOCHEMICAL REACTION KINETICS
BILE ACIDS
INTESTINAL ABSORPTION
CHOLESTEROL
ESTERIFICATION
BUFFERS
CARBON 14 COMPOUNDS
ENZYME ACTIVITY
HYDROLASES
INULIN
RATS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
ABSORPTION
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ENZYMES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
REACTION KINETICS
RODENTS
SACCHARIDES
STEROIDS
STEROLS
UPTAKE
VERTEBRATES
550501* - Metabolism- Tracer Techniques