Opposite responses of rabbit and human globin mRNAs to translational inhibition by cap analogues
Journal Article
·
· Biochemistry; (United States)
OSTI ID:5515531
The translational efficiency of an mRNA may be determined at the step of translational initiation by the efficiency of its interaction with the cap binding protein complex. To further investigate the role of these interactions in translational control, the authors compare in vitro the relative sensitivities of rabbit and human ..cap alpha..- and ..beta..-globin mRNAs to translational inhibition by cap analogues. They find that rabbit ..beta..-globin mRNA is more resistant to translational inhibition by cap analogues than rabbit ..cap alpha..-globin mRNA, while in contrast, human ..beta..-globin mRNA is more sensitive to cap analogue inhibition than human ..cap alpha..- and ..beta..-globin mRNAs is unexpected as direct in vivo and in vitro comparisons of polysome profiles reveal parallel translational handling of the ..cap alpha..- and ..beta..-globin mRNAs from these two species. This discordance between the relative translational sensitivities of these mRNAs to cap analogues and their relative ribosome loading activities suggests that cap-dependent events may not be rate limiting in steady-state globin translation.
- Research Organization:
- Univ. of Pennsylvania School of Medicine, Philadelphia
- OSTI ID:
- 5515531
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:22; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Glucocorticoids inhibit coordinated translation of. cap alpha. - and. beta. -globin mRNAs in Friend erythroleukemia cells
Translation of globin messenger RNA modified by benzo(a)pyrene 7,8-dihydrodiol 9,10-oxide in a wheat germ cell-free system
Translational control of ferritin synthesis: a study of repression using natural and synthetic mRNAs
Journal Article
·
Wed Nov 30 23:00:00 EST 1983
· Arch. Biochem. Biophys.; (United States)
·
OSTI ID:5267347
Translation of globin messenger RNA modified by benzo(a)pyrene 7,8-dihydrodiol 9,10-oxide in a wheat germ cell-free system
Journal Article
·
Thu Jan 24 23:00:00 EST 1980
· J. Biol. Chem.; (United States)
·
OSTI ID:5432293
Translational control of ferritin synthesis: a study of repression using natural and synthetic mRNAs
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6179495
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ENZYMES
ERYTHROCYTES
ESTERASES
EVEN-ODD NUCLEI
GENE REGULATION
GLOBIN
HYDROLASES
INHIBITION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RABBITS
RADIOISOTOPES
RETICULOCYTES
RIBOSOMES
RNA
RNA-ASE
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ENZYMES
ERYTHROCYTES
ESTERASES
EVEN-ODD NUCLEI
GENE REGULATION
GLOBIN
HYDROLASES
INHIBITION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RABBITS
RADIOISOTOPES
RETICULOCYTES
RIBOSOMES
RNA
RNA-ASE
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES