Evaluation of thrombogenicity of beta-propiolactone/ultraviolet (beta-PL/UV) treated PPSB in chimpanzees

Journal Article · Sun May 01 00:00:00 EDT 1983 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(83)90077-4· OSTI ID:5515487

Kotitschke, R; Stephan, W; Prince, A M; Brotman, B

The thrombogenicity of beta-PL/UV-treated PPSB (factor IX concentrate) was evaluated in chimpanzees. PPSB isolated from beta-propiolactone-treated and UV-irradiated plasma was injected into chimpanzees at a dose of approximately 100 units/kg body weight. An FDA licensed PPSB preparation served as the negative control, and a preparation containing activated as well as precursor clotting factors served as the positive control. 15 minutes, 1 h, 4 h, and 24 h after the PPSB application the following parameters were determined in the chimpanzee blood: factors II, VII, IX, X, VIII, fibrinogen, AT III, thrombin coagulase, Quick value, APTT and platelet count. Neither the untreated control preparation, nor the PPSB isolated from beta-propiolactone-treated and UV-irradiated plasma, showed signs of thrombogenicity in the chimpanzee model. The positive control indicated that the chimpanzee is a suitable model for the thrombogenicity testing of activated clotting factors.

• Research Organization: Biotest-Serum-Institut GmbH, Frankfurt, Main, Federal Republic of Germany
• OSTI ID: 5515487
• Journal Information: Thromb. Res.; (United States), Vol. 30:3
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BLOOD COAGULATION FACTORS
BIOLOGICAL MODELS
BLOOD PLASMA
LACTONES
MONKEYS
THROMBOSIS
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
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550900* - Pathology