sup 1 H and sup 51 V NMR studies of the interaction of vanadate and 2-vanadio-3-phosphoglycerate with phosphoglycerate mutase

Journal Article · Tue Mar 17 00:00:00 EST 1992 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00125a007· OSTI ID:5515305

Liu, S; Gresser, M J; Tracey, A S ^[1]

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1. Simon Fraser Univ., Burnaby, British Columbia (Canada)

The formation of complexes of vanadate with 2-phosphoglycerate and 3-phosphoglycerate have been studied using {sup 51}V nuclear magnetic resonance spectroscopy. Signals attributed to two 2,3-diphosphoglycerate analogues, 2-vanadio-3-phosphoglycerate and 2-phospho-3-vanadioglycerate, were detected but were not fully resolved from signals of inorganic vanadate and the anhydride formed between vanadate and the phosphate ester moieties of the individual phosphoglycerates. Equilibrium constants for formation of the two 2,3-bisphosphate analogues were estimated as 2.5 M{sup {minus}1} for 2-vanadio-3-phosphoglycerate and 0.2 M{sup {minus}1} for 2-phospho-3-vanadioglycerate. The results of the binding study are fully consistent with noncooperativity in the binding of vanadiophosphoglycerate to the two active sites of phosphoglycerate mutase (PGM). The results obtained here are in accord with these vanadate-phosphoglycerate complexes being much more potent inhibitors of phosphoglycerate mutase than either monomeric or dimeric vanadate. These results strongly support the view that phosphoryl transfer in this enzyme involves a pentacoordinate phosphate intermediate and suggests that the two active sites operate independently of each other.

• OSTI ID: 5515305
• Journal Information: Biochemistry; (United States), Vol. 31:10; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ISOMERASES
BIOCHEMICAL REACTION KINETICS
PHOSPHOLIPIDS
NUCLEAR MAGNETIC RESONANCE
NMR SPECTRA
PROTONS
VANADATES
VANADIUM 51
BARYONS
ELEMENTARY PARTICLES
ENZYMES
ESTERS
FERMIONS
HADRONS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
LIPIDS
MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
REACTION KINETICS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VANADIUM ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics