Immunoregulation by macrophages II. Separation of mouse peritoneal macrophages having tumoricidal and bactericidal activities and those secreting PGE and interleukin I

Journal Article · Wed Jun 01 04:00:00 EDT 1983 · J. Reticuloendothel. Soc.; (United States)

OSTI ID:5515203

Hopper, K E; Cahill, J M

Macrophage subpopulations having bactericidal or tumoricidal activities and secreting interleukin I (IL1) or prostaglandin E (PGE) were identified through primary or secondary infection with Salmonella enteritidis and separated by sedimentation velocity. Bactericidal activity was measured by (3H)-thymidine release from Listeria monocytogenes and tumoricidal activity by 51Cr-release from C-4 fibrosarcoma or P815 mastocytoma cells. Macrophages with bactericidal activity were distinguished from those with tumoricidal activity a) during secondary infection when cytolytic activity occurred only at days 1-4 post injection and bactericidal activity remained high throughout and b) after sedimentation velocity separation. Cytolysis was consistently greatest among adherent cells of low sedimentation velocity, whereas cells with bactericidal activity increased in size during the infection. Tumour cytostasis (inhibition and promotion of (3H)-thymidine uptake) differed from cytolysis in that the former was more prolonged during infection and was also detected among large cells. Secretion of immunoregulatory molecules PGE and IL1 occurred maximally among different macrophage subpopulations separated by sedimentation velocity and depending on the type of stimulus used in vitro. There was an inverse correlation between IL1 production and PGE production after stimulation with C3-zymosan or lipopolysaccharide (LPS). The development of immunity during infection may therefore be dependent upon the relative proportions of effector and regulatory macrophage subpopulations and the selective effects of environmental stimuli on these functions.

• Research Organization: Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St. Leonards, Australia
• OSTI ID: 5515203
• Journal Information: J. Reticuloendothel. Soc.; (United States), Journal Name: J. Reticuloendothel. Soc.; (United States) Vol. 33:6; ISSN JRSOD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYDROGEN ISOTOPES
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LIGHT NUCLEI
MACROPHAGES
MATERIALS
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PHAGOCYTES
PROSTAGLANDINS
RADIOISOTOPES
SALMONELLA
SOMATIC CELLS
TRITIUM
YEARS LIVING RADIOISOTOPES