Resistance of cytotoxic T lymphocytes to lysis by a clone of cytotoxic T lymphocytes

Journal Article · Fri May 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.10.3375· OSTI ID:5514310

Kranz, D M; Eisen, H N

To investigate how cytotoxic T lymphocytes (CTL) avoid killing themselves when they destroy target cells, the authors compared 20 different cell lines as target cells, including several CTL cell lines, for their susceptibility to lysis by CTL, measured by a standard /sup 51/Cr-release assay. Variations in recognition of this diverse set of target cells was circumvented by attaching to all of them a monoclonal antibody to the antigen-specific receptor of a cloned CTL cell line (clone 2C) and using the 2C cell line as the standard aggressor or effector cell. All of the nine tumor cell lines and the four noncytolytic T-helper cell lines tested as targets were highly susceptible to lysis by the aggressor CTL, but seven cytotoxic T-cell lines (six CTL and one T-helper cell line with cytotoxic activity) were largely resistant. These results, and the use of the lectin Con A as an alternative means for triggering CTL activity, point clearly to a level of resistance that could enable CTL to avoid their own destruction when they lyse target cells. The resistance of the cytolytic T cells did not appear to be accompanied by a similar resistance to complement-mediated lysis, indicating that mechanisms of CTL-mediated and complement-mediated lysis are not identical.

• Research Organization: Massachusetts Institute of Technology, Cambridge
• OSTI ID: 5514310
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
LYSIS
LYMPHOCYTES
TOXICITY
SPLEEN CELLS
IRRADIATION
TUMOR CELLS
CHROMIUM 51
CONCANAVALIN
ESTERASES
MICE
MONOCLONAL ANTIBODIES
AGGLUTININS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HEMAGGLUTININS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LECTINS
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture