Bioconversion of hemicellulose: Aspects of hemicellulase production by Trichoderma reesei QM 9414 and enzymic saccharification of hemicellulose

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260250419· OSTI ID:5513745

Dekker, F H

The growth of Trichoderma reesei QM9414 in shake flasks at 28/sup 0/C on hemicellulose substrates and bagasse resulted in rather low yields of hemicellulolytic enzymes (1.0-1.5 units/mL xylanase and 0.05-0.08 units/mL ..beta..-xylosidase). The influence of pH on the synthesis of ..beta..-xylosidase was greater than on the synthesis of xylanase. Both xylanase and ..beta..-xylosidase showed optimal activity at pH 4-5 and 55-60/sup 0/C. Xylanase was stable at pH 2-10 but was heat labile and totally inactivated after 1 h at 65/sup 0/C. Enzyme stability towards heat could be increased in the presence of bovine serum albumin. The ..beta..-xylosidase was more tolerant to heat, but stable over a pH range 2.5-6.0. The D-xylose inhibited both enzymes in a competitive manner. Hemicellulose (heteroxylan) was degraded to the extent of 30-40% within 24 h. The degree of hydrolysis decreased as the substrate concentration increased and increased with increased amounts of enzyme. Multiple enzyme doses resulted in increased saccharification in reduced times. The degree of hydrolysis was influenced by the amount of ..beta..-xylosidase present in the hemicellulolytic enzyme preparation. The ..beta..-xylosidase was demonstrated to play an important role in the overall conversion of heteroxyla into xylose that is analogous to the role of ..beta..-glucosidase in the saccharification of cellulose by cellulases.

• Research Organization: CSR Limited, Sugar Division, Sydney, 2001, NSW
• OSTI ID: 5513745
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. XXV:4; ISSN BIBIA
• Country of Publication: United States
• Language: English

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AGRICULTURAL WASTES
ALDEHYDES
BAGASSE
BIOMASS
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC WASTES
PENTOSES
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
WASTES
XYLANASE
XYLOSE