Effect of UV irradiation, toluidine blue, and environment on maternal haploid frequencies from the cross between Nicotiana tabacum and N. africana
Thesis/Dissertation
·
OSTI ID:5513167
Treating Nicotiana africana Merxm. pollen with three levels UV radiation prior to pollinating four cultivars of flue-cured tobacco (Coker 176, NC95, McN944 and PD4), Nicotiana tabacum produced 1,953 viable seedlings from an estimated total of 170,248 seeds, of which 1,667 were haploid and 286 were hybrids. Drenching N. tabacum flowers with toluidine blue 18 hours after pollination with normal N. africana pollen, yielded 511 viable seedlings from 70,613 seeds, of which 346 were haploid and 165 hybrids. Untreated pollen gave 548 viable seedlings from 56,291 seeds, comprising 341 haploids and 208 hybrids. Contrary to results from a previous histological study, in vivo pollen tube growth rate appears to be similar irrespective of pollen source or treatment, and fertilization seems to occur at about the same time as in the selfed control. From an estimated total of 803,854 seeds sown, 3,014 viable seedlings were obtained. Coker 176 gave significantly higher yields of haploids than the other three cultivars. Field grown plants produced more haploids than greenhouse grown plants. Further evidence was obtained to support selective chromosomal elimination as the mechanism governing the development of maternal haploids from this interspecific cross.
- Research Organization:
- Clemson Univ., SC (USA)
- OSTI ID:
- 5513167
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZO COMPOUNDS
AZO DYES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
DYES
GAMETES
GENETIC VARIABILITY
GERM CELLS
HAPLOIDY
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MUTATION FREQUENCY
NICOTIANA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PLOIDY
POLLEN
PRODUCTIVITY
RADIATION EFFECTS
TOLUIDINE BLUE
VIABILITY
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZO COMPOUNDS
AZO DYES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
DYES
GAMETES
GENETIC VARIABILITY
GERM CELLS
HAPLOIDY
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MUTATION FREQUENCY
NICOTIANA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PLOIDY
POLLEN
PRODUCTIVITY
RADIATION EFFECTS
TOLUIDINE BLUE
VIABILITY