'In vitro' studies on the interaction of rickettsia and macrophages. I. Effect of ultraviolet light on 'Coxiella burnetii' inactivation and macrophage enzymes: uv-inactivated 'C. burnetii'/macrophage enzymes. Interim report
The inactivation of Coxiella burnetii in suspension or in cultures of guinea pig peritoneal macrophages by ultraviolet (UV) light was studied. The effect of UV treatment on the activity of macrophage organelle marker enzymes and their subsequent equilibration in linear sucrose gradients was also determined. It was shown that UV treatment of 600 microwatts/sq cm for 15 sec at a distance of 10 cm inactivated C. burnetii, either in suspension (10 to the 8th power organisms/ML) or within guinea pig peritoneal macrophages. Similar UV treatment had little effect on the activity or equilibration of macrophage organelle marker enzymes in linear sucrose gradients. However, longer exposure caused considerable inactivatioin of these enzymes.
- Research Organization:
- Army Medical Research Inst. of Infectious Diseases, Frederick, MD (USA)
- OSTI ID:
- 5512159
- Report Number(s):
- AD-A-074768/3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
BIOLOGICAL RADIATION EFFECTS
MACROPHAGES
CELL CULTURES
CYTOPLASM
ENZYMES
FRACTIONATION
GUINEA PIGS
LABELLED COMPOUNDS
PERITONEUM
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
MAMMALS
MEMBRANES
MICROORGANISMS
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RODENTS
SEPARATION PROCESSES
SEROUS MEMBRANES
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)