Immobilization of enzyme on dimethyl-aminated nylon gels

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260271003· OSTI ID:5509187

Miyama, H; Kawate, M; Nosaka, Y

Miyama and co-workers have reported that dimethyl-aminated Nylon is a good material for the immobilization of enzyme after being quaternized. Also, they found a good correlation between the apparent Michaelis constant of the immobilized enzyme and the diffusion constant of the substrate in the matrix, and that between activity of the immobilized enzyme and interfacial free energy of the matrix. In their experiments, Nylon films of low degree of dimethylamination were used. In the present study, dimethylaminated Nylon of high degree of dimethylamination was quaternized by cationic oligomers having two functional groups. Thus, hydrogels were obtained, where the degree of cross-linking and the density of cation could be varied by changing the feeding amount and the length of cross-linking oligomers. These gels were then tested for the immobilization of urease and glucose oxidase. 9 references.

• Research Organization: Technological Univ. of Nagaoka, Japan
• OSTI ID: 5509187
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:10; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
CHEMICAL PREPARATION
COLLOIDS
DIFFUSION
DISPERSIONS
ENZYME ACTIVITY
GELS
IMMOBILIZED ENZYMES
MATERIALS
NYLON
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PERFORMANCE
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYAMIDES
POLYMERS
SUBSTRATES
SYNTHESIS
SYNTHETIC MATERIALS