Anaphylatoxin C3a induced mediator release from mast cells

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5508646

Herrscher, R; Hugli, T E; Sullivan, T J

The authors investigated the biochemical and functional consequences of the binding of highly purified human C3a to isolated rat serosal mast cells. C3a caused a dose-dependent (1-30 ..mu..M), noncytotoxic release of up to 64% (+/- 7 SEM) of the mast cell histamine content. C3a (10..mu..M) increased /sup 45/Ca/sup + +/ uptake 8.2- fold (+/- 2.2 SEM) above unstimulated control values within 10 minutes. Arachidonyl-diacylglycerol and arachidonyl-monoacylglycerol levels increased significantly within 2 minutes after C3a (10 ..mu..M) stimulation. Turnover of phosphatidylinositol, phosphatidic acid, and phosphatidylcholine were increased within 15 minutes. In contrast to antigen, C3a stimulation (10 ..mu..M) was not enhanced by exogenous phosphatidylserine, and was not inhibited by ethanol (100 ..mu..mM). C3a suppressed arachidonic acid (AA) release to 38% (+/- 9 SEM) below baseline, and did not cause PGD/sub 2/ formation. C3a and the desarginine form of C3a caused identical responses in all experiments. These studies indicate that C3a stimulation activates mast cell preformed mediator release in a manner very similar to antigen-IgE stimulation, but C3a suppresses free AA levels and does not stimulate PGD/sub 2/ synthesis.

• Research Organization: Research Institute of Scripps Clinic, La Jolla, CA
• OSTI ID: 5508646
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKALINE EARTH ISOTOPES
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
ARACHIDONIC ACID
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CALCIUM ISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHOLINE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ETHANOL
EVEN-ODD NUCLEI
GLYCEROL
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMIDAZOLES
INOSITOLS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPOTROPIC FACTORS
MAMMALS
MAST CELLS
MATERIALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SECRETION
SERINE
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES