Uptake and metabolism of (/sup 3/H)testosterone in the brain, pituitary gland and genital tract of the male cynomolgus monkey
To study the mechanism by which testosterone restores the sexual potency of castrated cynomolgus monkeys, two males (body weights 5.2 and 5.3 kg) were castrated and, 3 days later, injected with 3 mCi (/sup 3/H)testosterone ((/sup 3/H)T) as an intravenous bolus. After 30 min, males were killed and brains and samples of other tissues were rapidly removed and placed on ice. Samples were dissected from the right halves of the brain and homogenized. Purified cell nuclei were prepared and ether extracts were analyzed by reverse-phase HPCL. Generally, unchanged (/sup 3/H)T was the major form of radioactivity in brain and pituitary gland, but in cell nuclei from hypothalamus, preoptic area and amygdala, a large proportion (34 - 61%) was in the form of (/sup 3/H)estradiol ((/sup 4/H)E/sub 2/). Little or no (/sup 3/H)dihydrotestosterone ((/sup 3/H)DHT) was detected in cell nuclei from any brain region or from pituitary gland. However, (/sup 3/H)DHT was the major form (61 - 95%) of radioactivity in cell nuclei from glans penis, prostrate and seminal vesicles. In autoradiograms of the left halves of the same brains, the percentage of cells that accumulated radioactivity in their nuclei was high in specific regions of the hypothalamus, preoptic areas and amygdala. The authors conclude that the peripheral actions of T are mediated via DHT, but its central actions are dependent on unchanged T or on E/sub 2/ formed locally by aromatization.
- Research Organization:
- Emory Univ. School of Medicine, Atlanta, GA
- OSTI ID:
- 5507696
- Report Number(s):
- CONF-8604222-; TRN: 86-026665
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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TESTOSTERONE
METABOLISM
UPTAKE
AUTORADIOGRAPHY
BRAIN
CELL NUCLEI
ESTRADIOL
LIQUID COLUMN CHROMATOGRAPHY
MALE GENITALS
MONKEYS
PITUITARY GLAND
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMALS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
ENDOCRINE GLANDS
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PRIMATES
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
VERTEBRATES
550501* - Metabolism- Tracer Techniques