Repair of radiation-induced DNA double-strand breaks in isolated nuclei of Physarum polycephalum
Journal Article
·
· Radiat. Res.; (United States)
Radiation-induced DNA double-strand breaks are rejoined in homogenates and isolated nuclei of Physarum polycephalum. In agreement with results obtained previously for intact plasmodia, double-strand breaks are less extensively repaired in nuclei isolated from S-phase, as compared to G/sub 2/-phase, cultures. A corresponding propensity of DNA present in unirradiated nuclei of S-phase plasmodia toward breakage during incubation in vitro was observed. Repair of putative single-strand breaks, as ascertained from alkaline sucrose density-gradient centrifugation analysis, was not observed. The possible relationships between these phenomena are discussed.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH
- OSTI ID:
- 5504072
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 79:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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·
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·
OSTI ID:6452608
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Thesis/Dissertation
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Wed Dec 31 23:00:00 EST 1975
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OSTI ID:7083168
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL CYCLE
CELL NUCLEI
CENTRIFUGATION
COBALT 60
COBALT ISOTOPES
DNA
ELECTROMAGNETIC RADIATION
FUNGI
GAMMA RADIATION
IN VITRO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INVERTEBRATES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHYSARUM
PLANTS
PLASMODIUM
PROTOZOA
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SEPARATION PROCESSES
STRAND BREAKS
YEARS LIVING RADIOISOTOPES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL CYCLE
CELL NUCLEI
CENTRIFUGATION
COBALT 60
COBALT ISOTOPES
DNA
ELECTROMAGNETIC RADIATION
FUNGI
GAMMA RADIATION
IN VITRO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INVERTEBRATES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHYSARUM
PLANTS
PLASMODIUM
PROTOZOA
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SEPARATION PROCESSES
STRAND BREAKS
YEARS LIVING RADIOISOTOPES