A neoplastic epithelial duct cell line established from an irradiated human salivary gland

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · Cancer (Philadelphia); (United States)

DOI:https://doi.org/10.1002/1097-0142(19810801)48:3<745::AID-CNCR2820480314>3.0.CO;2-7· OSTI ID:5503083

Shirasuna, K; Sato, M; Miyazaki, T

Transformed epithelial cells were isolated by using tissue culture techniques from an irradiated human submandibular salivary gland which showed no neoplastic lesion. These cells, carrying colony-forming ability in semisolid agar, formed a semiconfluent monolayer with occasional tubular arrangement. All transformed clones were demonstrated by electron microscopic examination to be only one type of cells having fine structure similar to intercalated duct cells. Of six clones isolated, one clone with stable growth was cultured within the sponge matrix, resulting in formation of duct-like structure with mucinous eosinophilic substance. Moreover, inoculation of the cloned cells into nude mice resulted in a production of adenocarcinoma with solid and trabecular pattern. These findings indicate that a human intercalated duct cell line carrying tumorigenicity is established from a human submandibular salivary gland with an exposure to irradiation.

• OSTI ID: 5503083
• Journal Information: Cancer (Philadelphia); (United States), Journal Name: Cancer (Philadelphia); (United States) Vol. 48:3; ISSN CANCA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARCINOMAS
CELL CULTURES
DISEASES
GLANDS
IONIZING RADIATIONS
MAMMALS
MAN
MICE
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SALIVARY GLANDS
VERTEBRATES