Simple method for the preparation of phosphatidylcholine labelled at 2-acyl position
Journal Article
·
· Prep. Biochem.; (United States)
Phosphatidylcholine with a labelled acyl group at 2 position was prepared enzymatically from lysophosphatidylcholine and labelled fatty acids. Under optimal conditions, over 90% of (5,6,8,9,11,12,14,15-/sub 3/H) arachidonic acid, (9,10-/sub 3/H) palmitic acids, (1-/sub 14/C) linoleic acid or (9,10-/sub 3/H) oleic acid was incorporated into the phosphatidylcholine, and the incorporation was quantitative up to 500 nmol of (1-/sub 14/C) linoleic acid. Analysis of the labelled phosphatidylcholine formed indicated that radioactivity was incorporated exclusively at 2-acyl position.
- OSTI ID:
- 5502965
- Journal Information:
- Prep. Biochem.; (United States), Journal Name: Prep. Biochem.; (United States) Vol. 11:1; ISSN PRBCB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACYL RADICALS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHEMICAL PREPARATION
CHOLINE
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
LIVER
MAMMALS
MEASURING METHODS
METABOLISM
MICROSOMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
QUANTITATIVE CHEMICAL ANALYSIS
QUATERNARY COMPOUNDS
RADICALS
RADIOASSAY
RATS
RODENTS
SYNTHESIS
VERTEBRATES
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACYL RADICALS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHEMICAL PREPARATION
CHOLINE
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
LIVER
MAMMALS
MEASURING METHODS
METABOLISM
MICROSOMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHOLIPIDS
QUANTITATIVE CHEMICAL ANALYSIS
QUATERNARY COMPOUNDS
RADICALS
RADIOASSAY
RATS
RODENTS
SYNTHESIS
VERTEBRATES