Total sulfur concentration in tissues of control and cadmium-exposed rats by inductively coupled plasma atomic emission spectrometry
Total sulfur (S) concentration in biological samples was determined simultaneously with metal concentrations by inductively coupled plasma-atomic emission spectrometry (ICP). A 0.2 g portion of liver and other tissues were wet-digested with 1.0 ml mixed acid (HNO/sub 3/: HClO/sub 4/ = 5:1, v/v) at 130-150/sup 0/C. The solution was concentrated to about 0.1 ml and then diluted to 5.0 ml with double distilled water. Concentration of S was determined by ICP using ammonium sulfate as a standard S compound. Sulfur and other element concentrations in an NBS standard reference material (Bovine Liver SRM 1577) were within the certified values by this method. Concentrations of total S, cadmium (Cd), copper (Cu) and zinc (Zn) in the liver, kidney, spleen, lung, pancreas, and blood serum were compared between the control and Cd exposed rats. The three metal concentrations were increased significantly by Cd exposure. However, S concentration was not altered significantly in the liver and other tissues despite the extensive induction of metallothionein (MT) by the repeated Cd exposure. Metallothionein induced by the accumulated Cd (121 ..mu..g/g) and Zn (48 ..mu..g/g) in the liver was estimated to account for a maximum 7% of the total S by assuming that the increased metals were all bound to MT. Concentration of S in blood serum was decreased significantly by Cd loading.
- Research Organization:
- National Institute for Environmental Studies, Yatabe, Japan
- OSTI ID:
- 5502787
- Journal Information:
- Anal. Lett.; (United States), Vol. 19:7-8
- Country of Publication:
- United States
- Language:
- English
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ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ANIMAL TISSUES
QUANTITATIVE CHEMICAL ANALYSIS
CADMIUM
EMISSION SPECTROSCOPY
COPPER
SULFUR
ZINC
EXPERIMENTAL DATA
GLUTATHIONE
METALLOTHIONEIN
PLASMA
RATS
ANIMALS
BODY
CHEMICAL ANALYSIS
DATA
DRUGS
ELEMENTS
INFORMATION
MAMMALS
METALLOPROTEINS
METALS
NONMETALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SPECTROSCOPY
TISSUES
TRANSITION ELEMENTS
VERTEBRATES
400104* - Spectral Procedures- (-1987)