Bone marrow transplantation across major histocompatability barriers in mice. III. Treatment of donor grafts with monoclonal antibodies directed against Lyt determinants
We studied the effect of eliminating T cells from donor grafts of mice in a system in which bone marrow was transplanted across major histocompatibility barriers. BALB/c bone marrow (added as a source of hematopoietic stem cells) combined with equal volumes of spleen cells (added as a source of GVHD-promoting cells) was pretreated in vitro with monoclonal anti-Lyt-1.2 or Lyt-2.2 plus absorbed rabbit complement before injection into C57BL/6 total-body-irradiated recipients. Functional activity of anti-Lyt monoclonal antibodies was determined in CML assay. Treatment with anti Lyt-1.2 plus C did not have any anti-stem cell activity, as measured by CFU-S assay, and protected recipients from the onset of lethal GVHD. Treatment with Lyt-2.2 plus C also did not reduce CFU-S; however, mice receiving treated marrow did develop GVHD and were all dead by 2 mo, as were untreated control mice. Surviving ''anti-Lyt-1.2 + C chimeras'' demonstrated a high percentage of donor mononuclear cells in their peripheral blood. Similar results were obtained when C3H/HeN donor BMS was treated with monoclonal anti-Lyt-1.1 plus C and injected into C57BL/6 recipients. These findings show that monoclonal antibodies directed against determinants unrelated to Thy-1 can eliminate T cells in the presence of C and successfully protect transplanted mice from lethal GVHD. They also suggest that these anti-lyt antibodies may be useful tools in determining subpopulations of T cells that contribute to the development of GVHD.
- Research Organization:
- Univ. of Minnesota, Minneapolis
- OSTI ID:
- 5502460
- Journal Information:
- J. Immunol.; (United States), Vol. 128:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNOSUPPRESSION
RADIOINDUCTION
TRANSPLANTS
GRAFT-HOST REACTION
ANTIGEN-ANTIBODY REACTIONS
BONE MARROW
CHIMERAS
LYMPHOCYTES
MICE
SPLEEN CELLS
STEM CELLS
WHOLE-BODY IRRADIATION
X RADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
MOSAICISM
ORGANS
RADIATIONS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals