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DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
OSTI ID:5500966
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Operon fusions in Escherichia coli were obtained that showed increased beta-galactosidase expression in response to treatment with the DNA-damaging agent mitomycin C. These fusions were generated by using the Mud(ApR, lac) vector to insert the lactose structural genes randomly into the bacterial chromosome. Induction of beta-galactosidase in these strains, which carried fusions of lac to these din (damage-inducible) loci, was (i) triggered by UV light as well as by mitomycin C and (ii) abolished by either a recA- or a lexA- mutation. Similar characteristics of induction were observed when the lactose genes were fused to a prophage lambda promoter by using Mud(ApR, lac). These results indicate that E. coli contains a set of genes that, like prophage lambda genes, are expressed in response to DNA-damaging agents and regulated by the recA and lexA gene products. These din genes map at five bacterial loci. One din::Mud(ApR, lac) insertion results in a UV-sensitive phenotype and may be within the uvrA transcriptional unit.

Research Organization:
Massachusetts Inst. of Tech., Cambridge
OSTI ID:
5500966
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 77:5; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BACTERIA
BIOLOGICAL EFFECTS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
GALACTOSIDASE
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC MAPPING
GLYCOSYL HYDROLASES
HYBRIDIZATION
HYDROLASES
MAPPING
MICROORGANISMS
MITOMYCIN
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHOTOSENSITIVITY
RADIATIONS
SENSITIVITY
ULTRAVIOLET RADIATION