Oxygen-17 and deuterium nuclear magnetic resonance studies of lysozyme hydration

Journal Article · Thu May 15 04:00:00 EDT 1986 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(86)90534-5· OSTI ID:5498311

Lioutas, T S; Baianu, I C; Steinberg, M P

Oxygen-17 and deuterium NMR studies of lysozyme hydration are reported for a wide range of lysozyme concentrations, and the relationship between water activity and water mobility in the lysozyme-water system as determined by high-field NMR is examined. In a first approximation, the effect of lysozyme activity on hydration is considered to be small because of the relatively low charge on lysozyme at pH 7 and the absence of salt in the lysozyme solutions. Correlation times are determined for tightly bound water, weakly bound water, and multilayer or trapped water in lysozyme at 20 degrees C. Hydration numbers are also determined for these three different water populations interacting with lysozyme. Good agreement is found between the hydration numbers determined by 17O NMR and the calculations based on the D'Arcy and Watt analysis of water sorption isotherms for proteins that considered three major water populations in hydrated lysozyme. A molecular interpretation for the three components in the D'Arcy and Watt theory of sorption isotherms is also proposed on the basis of our NMR results. Previous proton NMR spin-echo results are shown to be consistent with our findings by 17O NMR and support the view that there are at least four regions of distinct hydration behavior of lysozyme which span the whole range from solutions to solid powders.

• Research Organization: Univ. of Illinois at Urbana-Champaign
• OSTI ID: 5498311
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 1; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400101* -- Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION ISOTHERMS
DEUTERIUM
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLYCOSYL HYDROLASES
HYDRATION
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROLASES
ISOTHERMS
ISOTOPES
LIGHT NUCLEI
LYSOZYME
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-ODD NUCLEI
OXYGEN 17
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
RESONANCE
SOLVATION
STABLE ISOTOPES
WATER