Heavy metal-induced gene expression in fish and fish cell lines
Two isoforms of metallothionein (MT) have been isolated from rainbow trout livers following CdCl/sub 2/ injections. These MTs have been identified by standard procedures and appear to be similar to mammalian MTs. Total RNA from such induced livers was shown to contain high levels of MT-mRNA activity when translated in cell free systems. This activity was demonstrated to be in the 8 to 10S region of a sucrose gradient. The RNA fractions also showed homology to a mouse MT-I cDNA probe. The exposure of rainbow trout hepatoma (RTH) cells to various concentrations of CdCl/sub 2/ and ZnCl/sub 2/ induced the expression of MT and MT-mRNA. Exposure of Chinook salmon embryonic (CHSE) cells to these metals, however, did not result in MT synthesis, suggesting that the MT genes have not become committed to transcription. Instead, an unknown low molecular weight (MW = 14 kDa) protein was induced. This metal-inducible protein (MIP) was capable of binding /sup 109/Cd and was stable to heating, while the binding of the metal to this protein was not. These characteristics have been reported for a protein induced in rainbow trout liver following environmental exposure to cadmium.
- Research Organization:
- Univ. of Calgary, Alberta
- OSTI ID:
- 5497952
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 65
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
CADMIUM CHLORIDES
BIOLOGICAL EFFECTS
METALLOPROTEINS
ZINC CHLORIDES
ANIMAL CELLS
FISHES
LIVER
MESSENGER-RNA
METALLOTHIONEIN
RECOMBINANT DNA
TROUT
TUMOR CELLS
ANIMALS
AQUATIC ORGANISMS
BODY
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DNA
GLANDS
HALIDES
HALOGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RNA
VERTEBRATES
ZINC COMPOUNDS
ZINC HALIDES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550601 - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques